Wilson's Disease Research Today is a free monthly online journal that collates and summarizes the latest research about Wilson's Disease, including details on treatment, causes, symptoms, medication.

A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents.

Somani BL, Ambade V

Department of Biochemistry, Armed Forces Medical College, Pune-411040, India. blsomani@gmail.com

OBJECTIVES: To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase. DESIGN AND METHODS: Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric. RESULTS: Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively. CONCLUSIONS: Norfloxacin method is a simple, inexpensive and automatable kinetic assay.

Published 23 April 2007 in Clin Biochem, 40(8): 571-4.
Full-text of this article is available online (may require subscription).

© 2005-2008 Wilson's Disease Research Today. All Rights Reserved.